Analysis of TIMP-1 (Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0586-LGZ)

Introduction

TIMPs are endogenous protease inhibitors that combine with matrix metalloproteinases (MMPs) at a 1:1 ratio to form complexes that regulate the activation or function of MMPs. TIMP-1 and TIMP-3 are glycoproteins, whereas TIMP-2 and TIMP-4 do not contain carbohydrates. TIMP-1, TIMP-2 and TIMP-4 are secreted proteins, whereas TIMP-3 is membrane-bound.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cardiovascular, Oncology & Cancer

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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