Analysis of TIMP-3 by ELISA (CAT#: STEM-MB-1554-LGZ)

Introduction

Tissue metalloproteinase inhibitor 3 (TIMP-3) is a kind of protein encoded by TIMP3 gene in human body, which belongs to the tissue inhibitor of metalloproteinase gene family. There are four members of the family: TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-3 is a 30 kDa glycoprotein secreted by many types of cells. TIMP-3 inhibits active MMP-mediated proteolysis by forming a non-covalent binary complex with the active site of MMP through its N-terminal domain. In addition, TIMP-3 is also an inflammatory factor inhibitor, which negatively regulates TACE and promotes the release of TNF-α, which in turn causes inflammatory response and vascular remodeling in the body. This gene mutation is associated with autosomal dominant Sorsby fundus dystrophy.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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