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Analysis of TNF-RII (Rat, Mouse) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0593-LGZ)

Introduction

TNF RII is also known as TNFRSF1B. This protein forms a heterocomplex with TNF RI, mediating the recruitment of two anti-apoptotic proteins c-IAP1 and c-IAP2 with E3 ubiquitin ligase activity.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cytokines & Chemokines, Oncology & Cancer, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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