Analysis of TNFRSF11B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1900-LGZ)

Introduction

Official Full Name: TNF receptor superfamily member 11b<br />Also known as: OPG; TR1; OCIF; PDB5<br />The protein encoded by this gene is a member of the TNF receptor superfamily. This protein is a decoy receptor secreted by osteoblasts and functions as a negative regulator of bone resorption. This protein specifically binds its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of mouse counterparts have also shown that this protein and its ligands play a role in lymph node organogenesis and vascular calcification. An alternatively spliced transcript variant of this gene has been reported, but its full-length nature has not been determined.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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