Analysis of TNFRSF1B/TNFR2/CD120b by ELISA (CAT#: STEM-MB-1590-LGZ)

Introduction

Tumor necrosis factor receptor II (TNF RII) is also known as tumor necrosis factor receptor superfamily member 1B (TNFRSF1B). This protein forms a heterocomplex with TNF RI, mediating the recruitment of two anti-apoptotic proteins c-IAP1 and c-IAP2 with E3 ubiquitin ligase activity. The function of IAPs in TNF receptor signaling is unknown, however c-IAP1 is thought to enhance TNF-induced apoptosis by ubiquitination and degradation of TNF receptor-associated factor 2 (TRAF2), which mediates anti-apoptotic signaling. Knockout experiments in mice showed that the protein plays a role in protecting neurons from apoptosis by stimulating antioxidant pathways.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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