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Analysis of TNFRSF25 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1256-LGZ)

Introduction

Official Full Name: TNF receptor superfamily member 25<br />Also known as: DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; GEF720; WSL-LR; PLEKHG5; TNFRSF12<br />The protein encoded by this gene is a member of the TNF receptor superfamily. This receptor is preferentially expressed in lymphocyte-rich tissues and may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-κB activity and regulate apoptosis. Signal transduction of this receptor is mediated by multiple adapter protein-containing death domains. Knockout studies in mice revealed a role for the gene in clearing autoreactive T cells in the thymus. Multiple alternatively spliced transcript variants encoding different isoforms of this gene have been reported, most of which are potential secreted molecules. Alternative splicing of this gene in B and T cells encounters programmed changes upon T cell activation, produces predominantly the full-length membrane-bound isoform, and is thought to be involved in the control of T cell activation-induced lymphocyte proliferation.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements