Analysis of TNFRSF4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1245-LGZ)

Introduction

Official Full Name: TNF receptor superfamily member 4<br />Also known as: OX40; ACT35; CD134; IMD16; TXGP1L<br />The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of ACOT7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1275-LGZ)
Analysis of C1orf167 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1267-LGZ)
Analysis of Duck Hepatitis Virus 3(DHV-3) by Real-Time PCR Method (CAT#: STEM-MB-2572-LGZ)
Analysis of OR2T34 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0362-LGZ)
Analysis of CACYBP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0815-LGZ)
Analysis of MARK1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0402-LGZ)
Analysis of VAMP5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1683-LGZ)
Analysis of Monkey Pox Virus (MPV) by Real-Time PCR Method (CAT#: STEM-MB-3009-LGZ)