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Analysis of TNNI2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2146-LGZ)

Introduction

Official Full Name: troponin I2, fast skeletal type<br />Also known as: DA2B; FSSV; DA2B1; fsTnI; AMCD2B<br />This gene encodes a fast-twitch skeletal muscle protein that is a member of the troponin I gene family and a component of the troponin complex, which includes troponin T, troponin C, and troponin I subunits . The troponin complex, together with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies suggest that this component is also present in vascular smooth muscle and may play a role in the regulation of smooth muscle function. In addition to muscle tissues, this protein is found in corneal epithelium, cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis, and mammalian gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This prote in It also suppresses tumor growth in human ovarian carcinoma. Mutations in this gene cause myopathy and distal arthrogryposis type 2B. Alternatively spliced transcript variants have been found for this gene.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements