Analysis of TOMM20 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0717-LGZ)

Introduction

Official Full Name: translocase of outer mitochondrial membrane 20<br />Also known as: MAS20; MOM19; TOM20<br />Enables protein-transporting ATPase activity and unfolded protein binding activity. Involved in protein targeting to mitochondrion. Located in mitochondria-associated endoplasmic reticulum membrane and mitochondrial outer membrane.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of TTC27 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1796-LGZ)
Analysis of H2AC18 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1027-LGZ)
Analysis of SP100 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1540-LGZ)
Analysis of DESI2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0922-LGZ)
Analysis of CAVIN3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2209-LGZ)
Analysis of C9orf163 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2010-LGZ)
Analysis of CENPS Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1283-LGZ)
Analysis of TMEM150A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1687-LGZ)