Analysis of Total Amino Acids (T-AA) by Enzyme-labeled Instrument (CAT#: STEM-MB-1443-LGZ)

Introduction

Sensitivity: 3.03 mmol/L
Detection range: 3.64-100 mmol/L
Precision: inter-lot difference of 6.5%, intra-lot difference of 4%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 640-660 nm

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Principle Applications Procedure Materials

Principle

Copper ions can be complexed with various amino acids to produce blue-green complexes, and the depth of color is proportional to the content of total amino acids at a given wavelength.

Applications

Suitable for the detection of total amino acid content in serum (plasma), urine, animal and plant tissues.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum (plasma), urine, animal and plant tissues

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