Analysis of Total Antioxidant Capacity (T-AOC) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1326-LGZ)

Introduction

Sensitivity: 0.62 U/mL
Detection range: 0.62-145.2 U/mL
Precision: inter-lot difference of 8.199999999999999%, intra-lot difference of 2.7%
Detection equipment: Ultraviolet-visible Spectrophotometer
Detection wavelength: 520 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

There are many antioxidant substances in the body, which can reduce the ferric ion to ferric ion, the latter can form a stable orange-red complex with phenoline substances, and its antioxidant capacity can be measured by colorimetry.

Applications

It is suitable for detecting the total antioxidant capacity in samples such as serum (plasma), saliva, urine, tissue, and cells.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: Serum (plasma), saliva, urine, tissue, cells

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