Analysis of Treponema Pallidum Subsp. Pallidum by Real-Time PCR Method (CAT#: STEM-MB-3187-LGZ)

Introduction

The bacterium Treponema pallidum subspecies pallidum ( T. pallidum) causes syphilis. It is a small, motile organism that is not visualized by light microscopy and cannot be cultured in vitro. The organism can be identified in CSF early in the course of disease, and neurosyphilis can occur at any time after acquisition of infection.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.