Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!
Treponema spp. is the main microorganism causing BDD in cattle and is often detected in BDD lesions. Treponema is spirochetal bacterium that can infect a wide range of hosts and tissues, causing a spectrum of diseases from syphilis in humans, to periodontal disease in both companion animals and humans and digital dermatitis (DD) in animals.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.