Analysis of Trichomonas Spp. by Real-Time PCR Method (CAT#: STEM-MB-3197-LGZ)

Introduction

Trichomonas spp. are mucosoflagellates that are isolated frequently from normal healthy nonhuman primates and are generally of no clinical significance. However, they may be pathogenic in immunodeficient hosts. Trichomonas spp. inhabit the crypts of the large intestine and are rarely associated with clinical signs or pathology.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.