Analysis of Trichostongylus Colubiformis by Real-Time PCR Method (CAT#: STEM-MB-3679-LGZ)

Introduction

In general, T. colubriformis is the species usually encountered in the Near and Middle East, whereas T. orientalis is the principal species in Asia. Trichostrongylus species and related genera are common parasites of herbivorous animals, including cattle, sheep, donkeys, goats, deer, and rabbits.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.