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Analysis of Triglyceride (TG) by Enzyme-labeled Instrument (CAT#: STEM-MB-1374-LGZ)

Introduction

Sensitivity: 0.14 mmol/L
Detection range: 0.14-10 mmol/L
Precision: inter-lot difference of 0.14-10 mmol/L, intra-lot difference of 4.1%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 490 -525 nm




Principle

Triglycerides (TG) can be hydrolyzed by Lipoprotein Lipase to glycerol and free fatty acids, and glycerol is treated with glycerol 3-phosphate and ADP by glycerol kinase (GK). Glycerol 3-phosphate produces hydrogen peroxide under the action of glycerol phosphate oxidase (GPO). When hydrogen peroxide is present in oxidase oxidase, it is catalyzed by peroxidase (POD). The reaction produces a red quinone compound, benzoquinimide phenazone, whose color is proportional to the TG content.

Applications

Used to detect the content of triglyceride in serum (plasma), animal tissue and cell samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum (plasma), animal tissue and cell samples
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