Analysis of TRIM34 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2181-LGZ)

Introduction

Official Full Name: tripartite motif containing 34<br />Also known as: IFP1; RNF21<br />The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, B-box type 1 and B-box type 2 domain, and a coiled-coil region. Expression of this gene is up-regulated by interferon. This gene is mapped to chromosome 11p15, where it resides within a TRIM gene cluster. Alternative splicing results in multiple transcript variants. A read-through transcript from the upstream TRIM6 gene has also been observed, which results in a fusion product from these neighboring family members.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Negishi Virus by Real-Time PCR Method (CAT#: STEM-MB-3614-LGZ)
Analysis of SHLD2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2055-LGZ)
Analysis of LCN6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2033-LGZ)
Analysis of Enteric Cytopathic Human Orphan Virus (ECHO) by Real-Time PCR Method (CAT#: STEM-MB-3841-LGZ)
Analysis of LCE1D Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1120-LGZ)
Analysis of NRBF2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2089-LGZ)
Analysis of Herpesvirus Simiae (HVS) by Real-Time PCR Method (CAT#: STEM-MB-3498-LGZ)
Analysis of ADAMTS13 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1967-LGZ)