Analysis of TRIM46 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0285-LGZ)

Introduction

Official Full Name: tripartite motif containing 46<br />Also known as: GENEY; TRIFIC<br />This gene encodes a tripartite motif (TRIM) family protein. The TRIM motif includes a zinc-binding domain, a RING finger region, a B-box motif, and a coil domain. TRIM46 has been reported to be involved in the proliferation of various cancer cells, including lung and breast cancer. It has also been shown to control neuronal polarity and axon specification through the formation of uniform microtubule bundles in axons.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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