Analysis of TRIM63 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0091-LGZ)

Introduction

Also known as: IRF; SMRZ; MURF1; MURF2; RNF28
This gene encodes a member of the RING family of zinc finger proteins found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z- and M-line lattices of myofibrils. This protein plays an important role in atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain proteins, myosin binding protein and muscle creatine kinase.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Inborn genetic diseases, Primary familial hypertrophic cardiomyopathy, Hypertrophic cardiomyopathy

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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