Analysis of TRIM68 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2177-LGZ)

Introduction

Official Full Name: tripartite motif containing 68
Also known as: SS56; GC109; SS-56; RNF137
The gene encodes a member of a triad-motif-containing protein family characterized by a "really interesting new gene" (RING) finger domain, a zinc-binding B-box motif, and a coiled-coil region. Members of this family function as E3 ubiquitin ligases and are involved in a wide range of biological processes. This gene regulates the activation of nuclear receptors such as the androgen receptor and has been implicated in the development of prostate cancer cells, and its expression increases with the downregulation of the microRNA. Furthermore, this gene is involved in the regulation of viral defense as a negative regulator of interferon-β. Alternative splicing results in multiple transcript variants.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Tensaw Virus by Real-Time PCR Method (CAT#: STEM-MB-3621-LGZ)
Analysis of KCND3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1148-LGZ)
Analysis of Burkholderia Cepacia by Real-Time PCR Method (CAT#: STEM-MB-2723-LGZ)
Analysis of PRAMEF7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1390-LGZ)
Analysis of Eurytrema Coelomaticum by Real-Time PCR Method (CAT#: STEM-MB-3642-LGZ)
Analysis of Canine Parvo Virus(CPV) by Real-Time PCR Method (CAT#: STEM-MB-2688-LGZ)
Analysis of SFT2D2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0664-LGZ)
Analysis of PRRC2C Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0584-LGZ)