Analysis of TRPM8 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1779-LGZ)

Introduction

Official Full Name: transient receptor potential cation channel subfamily M member 8<br />Also known as: TRPP8; LTRPC6; trp-p8; LTrpC-6<br />Predicted to enable ligand-gated calcium channel activity. Predicted to be involved in calcium ion transmembrane transport and positive regulation of cold-induced thermogenesis. Predicted to act upstream of or within several processes, including cellular calcium ion homeostasis; response to cold; and thermoception. Located in plasma membrane.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Avian Leukosis Virus (ALV) by Real-Time PCR Method (CAT#: STEM-MB-2847-LGZ)
Analysis of SDHB Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0084-LGZ)
Analysis of Dengue Virus (DENV) Type 2 by Real-Time PCR Method (CAT#: STEM-MB-4039-LGZ)
Analysis of UGT1A9 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1541-LGZ)
Analysis of La Crosse by Real-Time PCR Method (CAT#: STEM-MB-3606-LGZ)
Analysis of PUSL1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1396-LGZ)
Analysis of THEM4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0695-LGZ)
Analysis of BRCA1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2482-LGZ)