Analysis of Trypanosoma Evansi by Real-Time PCR Method (CAT#: STEM-MB-2956-LGZ)

Introduction

Trypanosoma evansi is a protozoan parasite that is the causative agent of the animal disease surra. The disease occurs in a wide area from the northern part of Africa through the Middle East to Southeast Asia; it is thought to have been introduced to the Americas in the 16th century and is now found in much of Latin America except the southernmost parts.<br />A real-time polymerase chain reaction (real-time PCR,or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.