Analysis of Trypanosoma Spp. by Real-Time PCR Method (CAT#: STEM-MB-2938-LGZ)

Introduction

Trypanosoma is a genus consisting of hemoflagellate protozoa that exist as obligatory parasites of plants, mammals and other animals (fish, birds, reptiles etc). Members of this genus, known as trypanosomes, are unicellular organisms whose life cycle is dependent on both vertebrate and invertebrate hosts.<br />A real-time polymerase chain reaction (real-time PCR,or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time),not at its end,as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter,which permits detection only after hybridization of the probe with its complementary sequence.