Analysis of TTC21B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1481-LGZ)

Introduction

Official Full Name: tetratricopeptide repeat domain 21B
Also known as: ATD4; THM1; FAP60; FLA17; SRTD4; CFAP60; IFT139; JBTS11; NPHP12; IFT139B; Nbla10696
This gene encodes a member of TTC21 family, containing several tetratricopeptide repeat (TPR) domains. This protein is localized to the cilium axoneme, and may play a role in retrograde intraflagellar transport in cilia. Mutations in this gene are associated with various ciliopathies, nephronophthisis 12, and asphyxiating thoracic dystrophy 4.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of PTCH2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0101-LGZ)
Analysis of THEM4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0695-LGZ)
Analysis of OR51L1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2353-LGZ)
Analysis of TRIM5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2180-LGZ)
Analysis of HBB-3'HS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2243-LGZ)
Analysis of LDB3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2073-LGZ)
Analysis of H2AC20 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1025-LGZ)
Analysis of LINC01593 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1463-LGZ)