Analysis of UGT1A7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1530-LGZ)

Introduction

Official Full Name: UDP glucuronosyltransferase family 1 member A7
Also known as: GNT1; UGT1; UDPGT; UGT1A; UGT1G; UGT-1A; UGT-1G; UGT1.1; UGT1.7; UGT1A1; UGT1-01; UGT1-07; hUG-BR1; UDPGT 1-7
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus encoding several UDP-glucosyltransferases. This locus consists of 13 unique alternating first exons followed by 4 common exons. Four alternating first exons are considered pseudogenes. Each of the remaining 9 5' exons could potentially splice with 4 common exons, resulting in 9 proteins with different n-termini and identical c-termini. Each first exon encodes a substrate binding site and is regulated by its own promoter. The enzyme encoded by this gene has moderate glucuronidase activity towards phenols.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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