Analysis of UTP25 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0731-LGZ)

Introduction

Official Full Name: UTP25 small subunit processome component<br />Also known as: DEF; DIEXF; C1orf107; DJ434O14.5<br />Enables RNA binding activity. Involved in several processes, including protein catabolic process; protein destabilization; and protein localization to nucleolus. Located in nucleolus.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Semen Phaseoli by Real-Time PCR Method (CAT#: STEM-MB-3939-LGZ)
Analysis of Border Disease Virus (BDV) by Real-Time PCR Method (CAT#: STEM-MB-2781-LGZ)
Analysis of ARHGEF2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0252-LGZ)
Analysis of Penicillium Cyclopium by Real-Time PCR Method (CAT#: STEM-MB-3027-LGZ)
Analysis of KDM5B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0342-LGZ)
Analysis of LOC110121236 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1586-LGZ)
Screening of Spike N501Y and HV69-70del Mutations by RT-qPCR (CAT#: STEM-MT-0025-LGZ)
Analysis of ABCG8 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1722-LGZ)