Analysis of VAMP3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1432-LGZ)

Introduction

Official Full Name: vesicle associated membrane protein 3<br />Also known as: CEB<br />Synaptobrevins/VAMPs, syntaxins, and 25-kD synaptosome-associated proteins are major components of protein complexes involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Due to its high homology to other known VAMPs, its wide tissue distribution and subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellulose protein. In platelets, this protein resides on a compartment that is not mobilized to the plasma membrane in response to calcium or thrombin stimulation.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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