Analysis of VEGFR-1/Flt-1 (Human) by ELISA (CAT#: STEM-MB-1261-LGZ)

Introduction

Human vascular endothelial growth factor receptor 1 (VEGFR-1 or Flt-1) is widely expressed in normal and pathological tissues and is involved in the pathogenesis of tumors and inflammatory diseases. In human cancers, VEGFR-1-mediated signaling is responsible for direct tumor activation and angiogenesis. VEGFR-1-mediated activation of non-malignant supporting cells, particularly stromal cells, dendritic cells, hematopoietic cells, and macrophages, may also be important for cancer pathogenesis. It has also been hypothesized that VEGFR-1 promotes the development of cancer metastasis through activation and pre-metastatic localization in distant organs of VEGFR-1-expressing bone marrow-derived hematopoietic progenitor cells.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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