Analysis of Visfatin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0629-LGZ)

Introduction

Visfatin, also known as nicotinamide phosphate nucleoside transferase (NAmPRT enzyme or NAMPT), formerly known as pre-B cell colony enhancer factor 1 (PBEF1), is an enzyme encoded by the NAMPT gene. Visfatin is abundant in visceral fat in both humans and mice, and its plasma levels increase during the development of obesity.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Metabolic, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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