Analysis of Wnt3a (Human, Mouse, Rat) by ELISA (CAT#: STEM-MB-1269-LGZ)

Introduction

The Wnt family has multiple secreted glycoproteins that play important roles in animal development. There are 19 Wnt genes in the human genome, which can encode Wnt proteins with different functions. Wnt members bind the Frizzled receptor family of seven transmembrane proteins and activate multiple signal transduction pathways. Aberrant activation of the Wnt signaling pathway is associated with various cancer types. The Wnt3a protein is palmitoylated and acts as a growth factor for hematopoietic stem cells.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Phosphoproteins, Intracellular Signaling

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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