Analysis of WRAP73 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1431-LGZ)

Introduction

Official Full Name: WD repeat containing, antisense to TP73<br />Also known as: WDR8<br />This gene encodes a member of the WD repeat protein family. The WD repeat is a minimally conserved region of approximately 40 amino acids, usually surrounded by gly-his and trp-asp (GH-WD), that may facilitate heterotrimeric or multiprotein complex formation. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Studies of related mouse proteins suggest that the encoded protein may play a role in the ossification process.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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