Analysis of Zika (ZIKV) and Chikungunya (CHIKV) Viruses by RT-qPCR (CAT#: STEM-MT-0065-LGZ)

Introduction

The re-emergence and spread of tropical viruses to new regions has caused a wave of concern around the world. In order to treat patients at an early stage and prevent the spread of the epidemic, early diagnosis is required for rapid and adequate detection.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Virus Detection

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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