The zinc ions in the sample form a colored complex with the 5-Br-PADAP in the reagent, the shade of which is proportional to the zinc ion concentration.
Applications
It is suitable for the determination of zinc content in serum, plasma, urine and milk.
Procedure
1. Prepare standard samples and experimental samples. 2. Add reaction reagents in order for reaction. 3. Measure the absorbance of each tube. 4. Make the mark curve and calculate the result.