Analysis of ZNF496 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0265-LGZ)

Introduction

Official Full Name: zinc finger protein 496<br />Also known as: NIZP1; ZFP496; ZSCAN49; ZKSCAN17<br />Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and protein self-association. Predicted to be involved in positive regulation of transcription, DNA-templated and regulation of transcription by RNA polymerase II. Predicted to act upstream of or within negative regulation of transcription by RNA polymerase II. Predicted to be located in nuclear body.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of LOC112840913 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1583-LGZ)
Analysis of C1orf131 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0241-LGZ)
Analysis of Arisaema Cum Bile by Real-Time PCR Method (CAT#: STEM-MB-3977-LGZ)
Analysis of ASCL2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2191-LGZ)
Analysis of Babesia Divergens by Real-Time PCR Method (CAT#: STEM-MB-2822-LGZ)
Analysis of TSPAN31 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2500-LGZ)
Analysis of CFAP45 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0845-LGZ)
Analysis of GATAD2B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0991-LGZ)