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Analyzing of human skin samples by transmission electron microscopy technology (CAT#: STEM-MIT-0018-LJX)

Introduction

Skin is the tissue on the surface of the body that surrounds the muscles. It is the largest organ in the human body. It is mainly responsible for protecting the body, sweating, feeling hot and cold and pressure. Skin covers the whole body and protects tissues and organs from physical, mechanical, chemical and pathogenic microorganisms.




Principle

Transmission electron microscopy (TEM) is to project the accelerated and concentrated electron beam onto a very thin sample, and the electron collides with the atoms in the sample and changes the direction, thus generating the stereo scattering Angle. The size of the scattering Angle is related to the density and thickness of the sample, so the image can be formed with different shades. The image can be enlarged, focused and displayed on imaging devices such as fluorescent screens, film and photosensitive coupling components. The resolution of transmission electron microscope is much higher than that of optical microscope, can reach 0.1~0.3nm, magnification of tens of thousands to millions of times. Therefore, transmission electron microscopy can be used to observe the fine structure of the sample.

Applications

Microscopic imaging in materials science or biology.

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Human skin samples

Notes

Pay attention to air humidity
Voltage needs to be stabilized
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