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Antibodies are immune globulins produced by the immune system in response to foreign molecules that enter the body. The foreign molecules are antigens. Their molecular recognition by the immune system leads to selective production of antibodies that are able to bind the specific antigen. Antibodies are produced by B lymphocytes and circulate throughout the blood and lymph where they bind to their specific antigen, enabling it to be cleared from circulation. Antibodies are divided into monoclonal antibodies and polyclonal antibodies, which are widely used in biology and medicine, disease prevention, medical diagnosis and scientific research.
The preparation of highly specific and high titer antibodies is the basis of experimental immunology techniques and the efficacy of antibody drugs. After the antibody is prepared, it needs to be further purified. The pure antibody is beneficial to preservation and to eliminate the influence of foreign proteins on the results. The quality of antibody reagents will directly affect the accuracy and stability of experimental data, and even the success or failure of the experiment.
Antibody preparation refers to the entire process of creating a usable specific antibody, including steps of immunogen preparation, immunization, hybridoma creation, collection, screening, isotyping, purification, and labeling for direct use in a particular method In the broad sense.
Antibodies prepared by different methods are often mixed with multiple heteroproteinas. In order to obtain antibodies with relatively single components or to use antibodies for specific purposes, antibody purification is required. The purity of antibody will directly affect the success of the experiment
STEMart provides you with a variety of antibody preparation and purification technology equipment or consumables to meet your various R&D and application needs. If you have any questions or requirements for antibody preparation and purification technology, please feel free to contact us.