Arbitrary PCR (AP-PCR) / RAPD (CAT#: STEM-MB-0223-WXH)

Introduction

The arbitrarily primed polymerase chain reaction (AP-PCR) is a PCR-based DNA fingerprinting technique using primers whose nucleotide sequence is arbitrarily chosen. This method has also been called random amplified polymorphic DNA (RAPD). <br />These methods have several advantages:<br />1.They can be used to produce quickly large numbers of discrete DNA fragments without prior sequence information;<br />2.Owing to the random nature of the process, the amplified fragments are likely to be evenly distributed across a genomic region;<br />3.Because they do not rely on the presence of species-specific repetitive sequences (e.g., Alu repeats), they can be used to analyze the genome of any species.