Assessement of the molecular integrity and stability of monoclonal antibodies by Dynamic light scattering (DLS) (CAT#: STEM-MB-0552-WXH)

Introduction

A monoclonal antibody (mAb, more rarely called moAb) is an antibody produced from a cell lineage made by cloning a unique white blood cell. All subsequent antibodies derived this way trace back to a unique parent cell.
Monoclonal antibodies are being used in the diagnosis and treatment of many diseases, including some types of cancer. They can be used alone or to carry drugs, toxins, or radioactive substances directly to cancer cells.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Dynamic Light Scattering (DLS) is an established and precise measurement technique for characterizing particle sizes in suspensions and emulsions. It is based on the Brownian motion of particles - this states that smaller particles move faster, while larger ones move slower in a liquid. The light scattered by particles contains information on the diffusion speed and thus on the size distribution.
The Dynamic Light Scattering (DLS) technique measures motion optically by recording the scattered light signal at a fixed angle. The particles are illuminated with a monochromatic, coherent light source (laser) and the light scattered by the particles is recorded.

Applications

DLS is used to characterize the size of various particles including proteins, polymers, micelles, Protein cages and virus-like particles, vesicles, carbohydrates, nanoparticles, biological cells, and gels.

Procedure

1. Sample preparation
2. Measurement by DLS instrument
3. Data analysis

Materials

• Dynamic Light Scattering (DLS) instruments (Photon Correlation Spectroscopy or Quasi-Elastic Light Scattering instruments)
• Dynamic Light Scattering Detector

Other recommended products

Characterize the size of virus-like particles by Dynamic light scattering (DLS) (CAT#: STEM-MB-0556-WXH)
Study of interactions between Agrin and small molecules by Dynamic light scattering (DLS) (CAT#: STEM-MB-0543-WXH)
Characterize the size of micelles by Dynamic light scattering (DLS) (CAT#: STEM-MB-0555-WXH)
Identifying the interactions during protein refolding reactions by Dynamic light scattering (DLS) (CAT#: STEM-MB-0553-WXH)
Characterization of PKR–RNA complex by Dynamic light scattering (DLS) (CAT#: STEM-MB-0542-WXH)
Study of solution conformation and dynamics of superhelical DNA by Dynamic light scattering (DLS) (CAT#: STEM-MB-0554-WXH)
Characterize the size of vesicles by Dynamic light scattering (DLS) (CAT#: STEM-MB-0557-WXH)
Homogeneity of viral RNA molecules by Dynamic light scattering (DLS) (CAT#: STEM-MB-0539-WXH)