BAC Library Construction to study genome sequence (CAT#: STEM-MB-0037-WXH)

Introduction

Bacterial Artificial Chromosome (BAC) acts as a vector to artificially carry DNA into the cell of a bacterium, such as Escherichia coli, to make a BAC clone. In general, BAC clones can carry inserts of DNA up to 200,000 base pairs long. Recently, BAC is used to create BAC libraries which are preferable for genome sequencing, physical maps constructing, and gene cloning.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Study the genome sequence of a particular gene
• Determine the complete genome sequence of a given organism
• Construct physical maps

Procedure

1.Preparation of high-molecular-weight DNA.
2.Partial digestion of high molecular weight DNA and size selection.
4.Ligation and electroporation.
5.Isolation of BAC DNA and estimation of insert size.
6.Library pooling and PCR screening.

Notes

Customer provides adequate amount of fresh samples (animal or plant tissue or genomic DNA)

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