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Baculovirus-insect Cell Protein Expression System (CAT#: STEM-MB-0123-WXH)

Introduction

The baculovirus-insect cell expression system has the advantages of economy, easy scale-up and high expression level, and is one of the most widely used eukaryotic expression systems. Its post-translational modification is similar to mammalian cells, and it is suitable for expressing some proteins that may have negative effects on mammalian host cells, such as kinases and toxic proteins. The baculovirus expression system is a eukaryotic expression system that has many advantages in protein expression, such as accommodating a large number of DNA inserts, relatively high expression levels, and eukaryotic protein modification similar to mammalian cells. The baculovirus expression system is the most commonly used expression system for structural analysis.




Applications

• Production of Large-Scale Recombinant Proteins.
• Suitable for expressing higher eukaryotic proteins, secreted proteins, multi-protein complexes, etc.

Procedure

1. Gene synthesis and codon optimization
2. Vector construction
3. Virus packaging and amplification
4. Protein expression and purification
5. Mass expression and purification

Notes

Customers provide sequence information or plasmids of target proteins.

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