Bicinchoninic Acid (BCA) Assay

The bicinchoninic acid (BCA) assay is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL). The total protein concentration is exhibited by a color change of the sample solution from green to purple in proportion to protein concentration, which can then be measured using colorimetric techniques.

Fig.1 Schematic diagram of bicinchoninic acid (BCA) assay principle.

• Reagents
  Serially dilute bovine serum albumin (BSA) standards.
  Prepare BCA working solution.
• Assay
  Add each dilution of protein standard and test protein sample to a microplate or test tube.
  Add BCA working solution separately.
  After sealing, incubate at 37°C for 30-60 minutes.
• Analysis
  Measure the absorbance of the sample at 562 nm.
  Calculate the protein concentration of the sample based on the standard curve and the sample volume used.

• This method is simple and fast, four times faster than the lowry method.
• The sensitivity is high, the minimum detection amount is 0.5µg.
• It has good stability and good linearity in the range of 20µg/ml~2000µg/ml.
• It is economical and practical, and the volume of the sample to be tested can be 1µl~20µl.

• Widely used for protein concentration determination.

• When using BCA protein determination method, the color will be deepened with the extension of time, and the speed of color reaction is related to the temperature, so it is necessary to maintain the timing and constant temperature to ensure accurate quantification.
• Low temperature or long-term storage, if it is found that BCA reagent A or B precipitation occurs. Incubate at 37ºC and stir to dissolve fully. If bacterial contamination is found, it should be discarded to avoid affecting the experimental results.
• The experimenter should operate the specification to improve the accuracy of sample loading.
• The corresponding standard curve should be made for each measurement, because the color reaction is related to the change of temperature and time, and the standard curve should be made every time for accurate protein quantification.
• For your safety and health, please wear lab coats and disposable gloves.

Microcentrifuges

Whether in molecular or cell biology, there are countless workflow steps that would not be possible without the use of a microcentrifuge. Because it is used to spin various samples at high speed. For example, nucleic acids or proteins can be precipitated from solution, small water samples can be microfiltered and the liquid collected at the bottom of the tube to avoid waste.

Pipettes and Pipette Tips

Pipette is a measuring tool that moves liquid from the original container to another container within a certain range. It is widely used in clinical diagnostic laboratories, biotechnology laboratories, pharmaceutical and chemical laboratories, environmental laboratories, and food laboratories. We provide a variety of pipettes for you to choose to use in a variety of experimental research.

Laboratory Incubators

An incubator is a device used to grow and maintain microbiological cultures or cell cultures. It is made up of a chamber with a regulated temperature. An incubator maintains optimal temperature, humidity, and other conditions such as the CO2 and oxygen content of the atmosphere inside. Incubators are essential for much experimental work in cell biology, microbiology, and molecular biology.

Microplates and Plate Seals

A variety of microplates are available for a wide range of applications. The correct selection of a microplate can improve assay performance in a number of ways. Whether you’re looking for a permanent seal for long term storage or seals to enable your samples to breathe, find your favourite or next best seal right here, right now.

Microplate Readers

A microplate reader is a specialized spectrophotometer designed to read the results of a technique used to determine the presence of specific antibodies or antigens in a sample. Our microplate readers have empowered life science researchers to advance protein and cell biology―breaking the barriers to novel, landmark discoveries.

Spectrophotometers

Spectrophotometer is an optical instrument that measures the intensity of light in relation to the wavelength. It can measure just about anything such as: plastics, paper, liquid, metal, fabric and so on. A spectrophotometer is used to check for the quality of product in a production facility and identify and quantify microscopic samples in analytical laboratories.

Lab Coats

Lab workers must, at a minimum, wear lab coats and safety glasses while in a lab where chemicals are handled. keeping the wearer clean and offering some protection from light liquid splash and other workplace hazards. Shorts and sandals may not be worn into the lab even under a lab coat.

Lab Gloves

Hand protection is especially important for lab workers, as a good protective glove will provide high levels of protection from a number of different dangers. We have a range of Laboratory Gloves that are suitable for use in labs for applications such as experiments, chemical synthesis and clinical trials.

STEMart provides you with a variety of bicinchoninic acid (BCA) assay equipment to meet your various R&D and application needs. If you have any questions or requirements for bicinchoninic acid (BCA) assay equipment, please feel free to contact us.