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BIO-RAD IQ5, 15 - 100µl In Pleasanton, CA, USA (CAT#: STEM-PCREPO-0095-YJL)

Highlights

–Multiplexing of up to 5 fluorophores in each reaction vessel conserves precious samples and reagents
–Real-time PCR thermal gradient enables simple, rapid assay optimization
–Intuitive software for protocol setup and data analysis shortens time to results

Cat Number: STEM-PCREPO-0095-YJL

Application: Used for many qualitative and quantitative applications, including gene expression analysis, microRNA analysis for identification of cancer biomarkers, single nucleotide polymorphism (SNP) genotyping, copy number variation (CNV) analysis, and even protein analysis

Model: IQ5

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Description

BIO-RAD IQ5, 15 - 100µl In Pleasanton, CA, USA, model IQ5, is manufactured by Bio-Rad biotechnology & life science.
The iQ5 real-time PCR detection system offers a fresh design for Bio-Rad’s new generation of real-time PCR instrumentation. Like its predecessors, the iCycler iQ and MyiQ systems, the iQ5 optical system is a modular upgrade to the iCycler thermal cycler with 96-well reaction block. This powerful new system offers five-target analysis capabilities for multiplex PCR, coupled with a completely redesigned software package.
The iQ5 optical system software offers a full suite of enhanced data collection and data analysis options, including tools for gene expression analysis that measure relative quantities without a standard curve. Regardless of your PCR application, you will find that the iQ5 real-time PCR detection system is the ultimate system for value and flexibility.

Specification

Condition: Used
Optics: Excitation laser
488-nm blue direct diode laser, nominal power: 16 mW
640-nm red direct diode laser, nominal power: 36 mW
Beam size: 9± 3 x 67 ±5 µm
Laser beam alignment: Fixed and spatially separated alignment of all lasers with the cuvette flow cell
Hardware Specifications
Maximum sample size: up to 28 x 36 cm
Maximum image area: up to 26 x 35 cm
Maximum image area: (For standard UV-excited gels) 25 x 26 cm
Excitation source: Epi-white light and trans-UV (302 nm) are standard (optional 365 nm lamp available); optional trans-white conversion screen and XcitaBlue™ UV/blue conversion screen available
Illumination control: 5 modes (trans-UV, epi-white, and no illumination for chemiluminescence are standard); trans white and XcitaBlue conversion screens are optional
Detector: Supercooled CCD
Pixel size (H x V): 6.45 x 6.45 µm
Cooling system: Peltier
Camera cooling temperature: -30°C controlled
Filter selector: 3 positions (2 for filters, 1 without filter, for chemiluminescence)
Emission filters: 1 included (standard), 3 optional
Dynamic range: >4.0 orders of magnitude
Pixel density (gray levels): 65,535
Dynamic flat fielding: Application specific, for all applications
Instrument size (L x W x H): 36 x 60 x 96 cm
Instrument weight: 32 kg
Operating Ranges
Operating voltage: 110/115/230 V AC nominal
Operating temperature: 10–28°C (21°C recommended)
Operating humidity: <70% noncondensing

Features

–Multiplexing of up to 5 fluorophores in each reaction vessel conserves precious samples and reagents
–Real-time PCR thermal gradient enables simple, rapid assay optimization
–Intuitive software for protocol setup and data analysis shortens time to results
–Diverse data analysis and presentation options provide a comprehensive view of your assay result
–Flexible allelic discrimination module allows easy-to-use, automatic sample identification in genotyping experiments
–Embedded tools for end-point fluorescence analysis enables simple qualitative analysis of sample abundance in single- or multicolor assays
–Robust, reliable thermal cycling performance delivered by the iCycler thermal cycler

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