Biotinylated Recombinant Human Her3/ErbB3 Protein , His-Avi Tag, Yisheng (CAT#: STEM-CT-0259-ZJF)

The Biotinylated Recombinant Human Her3/ErbB3 Protein , His-Avi Tag manufactured by Yisheng is suitable for detecting Her3/ErbB3-positive cells in research.
ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB3 is unique in that it contains a defective kinase domain. It is expressed in various cell types, and its activation involves heterodimerization with other ErbB family members. The cytoplasmic domain of ErbB3 contains binding motifs for the regulatory subunit of phosphoinositide 3-kinase (PI3K), suggesting a role in PI3K signaling.

Synonyms: ErbB3; ErbB-3; HER3; HER3c-erbB-3; LCCS2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; ERBB3
Uniprot No.: P21860.1
Source: Recombinant Biotinylated Human Her3/ErbB3 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Ser20-Thr643.
Molecular Weight: Approximately 71.6 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.
Purity: > 95% as determined by SDS-PAGE and HPLC.
Activity: ELISA Data: Immobilized Biotinylated Human Her3 at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-Her3 Antibody, hFc Tag with the EC50 17.6 ng/mLdetermined by ELISA.
Endotoxin: < 1.0 EU per 1μg of the protein by the LAL method.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Shipped with ice pack.
Stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 ℃ under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
For safety and health, please wear lab coats and disposable gloves for operation.

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