Blood analysis by Raman spectroscopy (CAT#: STEM-ST-0074-WXH)

Introduction

Blood analysis is frequently performed for medical diagnosis. Approximately 600 million cholesterol tests are performed annually worldwide to assess the risk and severity of cardiovascular disease. More than 100 million diabetics are advised to monitor their glucose levels several times each day. There is a great interest in optical measurement that would permit simultaneous analysis of multiple components (analytes) in whole blood without the need for conventional sample processing, such as centrifuging and adding reagents.

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Principle Applications Procedure Materials Notes Related Products

Principle

Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions.
The principle behind Raman spectroscopy is that the monochromatic radiation is passed through the sample such that the radiation may get reflected, absorbed, or scattered. The scattered photons have a different frequency from the incident photon as the vibration and rotational property vary.

Applications

• Analysis of biocompatibility of a material.
• Analysis of nucleic acids.
• Study of interactions between drugs and cells.
• Photodynamic therapy (PDT).
• Analyzing metabolic accumulations of a substance or compounds.
• Diagnosis of disease.
• Analysis of individual cells.
• Cell sorting applications.
• Analyzing the features of biomolecules.
• Study of bone structure.

Procedure

1. Preparation of samples
2. Determine instrument parameters
3. Perform background scan
4. Test the sample
5. Data analysis

Materials

• Raman Spectrometer
• Raman Imaging Microscope

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