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Cell movement is one of the body's metabolism and basic life characteristics. Directed migration of cells is closely related to embryogenesis, neural development, immune response, and organ maturation. Many major human diseases and their treatments, such as tumor metastasis, nerve repair, regeneration of stem cell functions, etc., are closely related to cell movement.
The boyden chamber method is currently the most commonly used method for detecting cell motility. Cells are added to the upper chamber and serum or other chemokines are added to the lower chamber to induce cells to migrate to the lower chamber. The number of migrated cells was manually counted by staining to assess cell migration.