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CaptureSelect™ FcXL PhyTip® Columns (1 mL Volume) Containing 10 µL Affinity Media Per Column, PTT-91-10-32, Tecan (CAT#: STEM-C-4145-LGZ)

Highlights

PhyNexus, now part of Biotage, developed PhyTip® columns in 2002 and has supported researchers in automated protein purification since then. PhyTip columns purify samples through repeated back and forth cycles, a process known as dual-flow chromatography that allows for complete binding interactions during each purification step.

IgGs recovery 70 - 90%

Cat Number: STEM-C-4145-LGZ

Application: For purification of recombinant human IgG, Fc fusion proteins and plasma derived IgG.

Model: PTT-91-10-32

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Description

PhyTip columns with CaptureSelect FcXL affinity matrix can be used for the purification of recombinant human IgG, Fc fusion proteins and plasma-derived IgG by binding to the CH3 domain of all human IgG subclasses (I, II, III and IV).

Affinity ligands for ThermoFisher's CaptureSelect™ media are based on recombinant antibody fragments of only 14 kDa in size and can be adapted for highly selective binding to certain moieties or antibodies and antibody fragments.

Specification

Pack Size: 96

Features

PhyNexus, now part of Biotage, developed PhyTip® columns in 2002 and has supported researchers in automated protein purification since then. PhyTip columns purify samples through repeated back and forth cycles, a process known as dual-flow chromatography that allows for complete binding interactions during each purification step.

IgGs recovery 70 - 90%
Less protein loss during purification due to maximized sample binding. The result is very high sample recovery.

Sample concentration and purification in one
Our unique packed resin bed column design results in the entire sample being bound to a small resin bed volume. Purified samples can be eluted in small volumes, resulting in highly concentrated samples.

IgG purification for 15 minutes
Efficient purification via PhyTip columns allows for a complete purification protocol of 96 samples in as little as 15 minutes.

Compatible with all major liquid handling systems
Our PhyTip columns are compatible with all major liquid handling platforms, enabling you to use your existing systems, which you may already have in your laboratory for automated protein purification. If you do not have a system, we can provide an automated solution for protein purification with PhyTip columns.

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