Carbohydrate Immobilization Kit, For 5 x 2ml Column, 786-807, G-BIOSCIENCES (CAT#: STEM-C-3111-LGZ)

Carbohydrate coupling resins (hydrazine resins) are designed for simple and efficient coupling of glycoproteins to solid agarose supports by oxidizing sugar groups. The coupling is through the hydrazine group which is immobilized to the sepharose beads. This resin is ideal for immobilizing polyclonal antibodies whose Fc domains are rich in carbohydrates. The location of the Fc domain ensures that the binding site of the antibody is kept away from the resin for optimal binding and reduced steric hindrance.

Glycoprotein sugar components are first oxidized with sodium metaiodate to convert cis-diol groups into reactive aldehydes. These aldehydes react spontaneously with the hydrazine groups on the carbohydrate coupling resin to form stable hydrazone linkages (see figure). The long spacer (23Å) reduces steric hindrance and ensures better binding of proteins and antibodies during affinity purification. The resulting affinity resin can be reused at least 10 times without significant loss of activity.

Carbohydrate immobilization kit provides carbohydrate coupling resin column for preparing 2ml affinity column, coupling catalyst, SpinOUT™ GT-600 desalting column for removing oxidizing agent, oxidizing reagent (sodium metaiodate) and related buffer .

Size: For 5 x 2ml column

Hydrazide activated resin for binding reactive aldehydes
Couple 1-5mg glycoprotein/ml resin
Long Spacer Arm (23Å)
Gentle coupling conditions

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