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Catalytic Heterogeneity in Lactate Dehydrogenase (CAT#: STEM-AC-0075-WXH)

Introduction

Lactate dehydrogenase is an enzyme that the body uses during the process of turning sugar into energy for your cells to use. LDH is found in many of the body's tissues and organs, including the muscles, liver, heart, pancreas, kidneys, brain and blood cells.




Principle

The temperature jump method is a technique used in chemical kinetics for the measurement of very rapid reaction rates.
The T-Jump technique provides a means to follow fast reactions with half-lives of just a few microseconds. The reaction volume is prepared so that it is in equilibrium and then rapidly perturbed by a rapid change in temperature. There is a new equilibrium constant at the higher temperature, but the initial concentrations are balanced for the lower temperature. The system therefore relaxes and the reaction proceeds until the concentrations have reached their new equilibrium values.

Applications

Used in chemical kinetics for the measurement of very rapid reaction rates

Materials

Temperature Jump System

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