Boronate Affinity Chromatography Technology

Boronate affinity chromatography (BAC) is a unique mode of affinity chromatography. It relies on the pH‐dependent, covalent and reversible interactions between boronic acid ligands and cis ‐diol‐containing compounds. This chemistry endows BAC with the unique capability for selective recognition, capture, isolation, and enrichment of cis ‐diol‐containing biomolecules. Cis-diol-containing biomolecules are an important class of compounds, including glycoproteins, glycopeptides, ribonucleosides, ribonucleotides, saccharides, and catecholamines. These biomolecules play essential roles in many life-related processes. Because cis-diol-containing biomolecules are important target molecules in current research frontiers such as proteomics, metabolomics, and glycomics, BAC and boronate affinity materials have gained rapid development and found increasing applications in recent decade.