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Brillouin spectroscopy has emerged as a promising modality to noninvasively probe the mechanical properties of biologically relevant materials. Due to its all-optical, contact-free, and label-free nature, Brillouin spectroscopy allows for in vivo 3D mapping of longitudinal elastic modulus distribution within cells and tissues, even in settings where physical contact is not possible, such as in 3D matrices or microfluidics channels, providing sufficient sensitivity to detect and monitor relevant biomechanical changes.